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anti il6r alpha antibody  (R&D Systems)


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    R&D Systems anti il6r alpha antibody
    Th17 cultures containing IL‐21+ TGF‐β1 (Th17.d) develop independently of T cell–intrinsic IL‐6 . WT or Il6 KO CD4 + T cells were differentiated under Th17.d conditions for 7 days, and Il17A expression was assessed by ELISA (A) and qPCR (B). Responsiveness to CD8 + T‐cell‐mediated suppression was evaluated in coculture assays (C). In parallel, WT cells were cultured with or without <t>anti–IL6R</t> antibody and subjected to the same functional readouts (D–F). Data are from three independent experiments ( n = 6 per group); each point represents an individual donor. Error bars indicate SEM. Statistical analysis was performed using paired or unpaired Student's t ‐test (* p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; ** p ≤ 0.0001).
    Anti Il6r Alpha Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+human+il6r+antibody/Human+IL-6R+alpha+Antibody/pmc12929703-224-11-14
    Average 94 stars, based on 25 article reviews
    anti il6r alpha antibody - by Bioz Stars, 2026-10
    94/100 stars

    Images

    1) Product Images from "CD4 + T‐Cell‐Intrinsic IL‐6 Is Critical for Th17 Differentiation and Dampened Responsiveness to CD8 + T Cell‐Mediated Suppression"

    Article Title: CD4 + T‐Cell‐Intrinsic IL‐6 Is Critical for Th17 Differentiation and Dampened Responsiveness to CD8 + T Cell‐Mediated Suppression

    Journal: European Journal of Immunology

    doi: 10.1002/eji.70150

    Th17 cultures containing IL‐21+ TGF‐β1 (Th17.d) develop independently of T cell–intrinsic IL‐6 . WT or Il6 KO CD4 + T cells were differentiated under Th17.d conditions for 7 days, and Il17A expression was assessed by ELISA (A) and qPCR (B). Responsiveness to CD8 + T‐cell‐mediated suppression was evaluated in coculture assays (C). In parallel, WT cells were cultured with or without anti–IL6R antibody and subjected to the same functional readouts (D–F). Data are from three independent experiments ( n = 6 per group); each point represents an individual donor. Error bars indicate SEM. Statistical analysis was performed using paired or unpaired Student's t ‐test (* p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; ** p ≤ 0.0001).
    Figure Legend Snippet: Th17 cultures containing IL‐21+ TGF‐β1 (Th17.d) develop independently of T cell–intrinsic IL‐6 . WT or Il6 KO CD4 + T cells were differentiated under Th17.d conditions for 7 days, and Il17A expression was assessed by ELISA (A) and qPCR (B). Responsiveness to CD8 + T‐cell‐mediated suppression was evaluated in coculture assays (C). In parallel, WT cells were cultured with or without anti–IL6R antibody and subjected to the same functional readouts (D–F). Data are from three independent experiments ( n = 6 per group); each point represents an individual donor. Error bars indicate SEM. Statistical analysis was performed using paired or unpaired Student's t ‐test (* p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; ** p ≤ 0.0001).

    Techniques Used: Expressing, Enzyme-linked Immunosorbent Assay, Cell Culture, Functional Assay

    Related Articles

    other:

    Article Title: Anti-CD137 antigen-binding molecule and utilization thereof
    Article Snippet: After the plate was left to stand for one hour at room temperature, a biotin-labeled anti-mouse IgG antibody (Southern Biotechnology Associates, Inc) was added and left to stand at room temperature for one hour.

    Clinical Proteomics:

    Article Title: PK/PD analysis of a novel pH-dependent antigen-binding antibody using a dynamic antibody-antigen binding model.
    Article Snippet: Previously, we have reported novel engineered antibody with pH-dependent antigen-binding (recycling antibody), and with both pH-dependent antigen-binding and increased FcRn-binding at neutral pH (sweeping antibody).. The purpose of this study is to perform PK/PD predictions to better understand the potential applications of the antibodies as therapeutics.. To demonstrate the applicability of recycling and sweeping antibodies over conventional antibodies, PK/PD analyses were performed.

    Article Title: Anti-CD137 antigen-binding molecule and utilization thereof
    Article Snippet: .. Murine plasma samples diluted 50 fold were mixed with a SULFO-TAG (Meso Scale Diagnostics, LLC)-labeled anti-human IL6R antibody (R&D Systems), a biotin-labeled anti-human IL6R antibody (R&D Systems), and excessive anti-human IL6R antibody tocilizumab (in-house manufactured product), and the mixture was incubated overnight at 37° C. After the mixture was added to the blocked Streptavidin-coated standard 96-well plate (Meso Scale Diagnostics, LLC), followed by addition of Read buffer, the reaction was measured on SECTOR Imager 6000 (Meso Scale Diagnostics, LLC). .. SOFTmax PRO (Molecular Devices) was used to calculate concentrations of the soluble antigen in murine plasma.

    Incubation:

    Article Title: PK/PD analysis of a novel pH-dependent antigen-binding antibody using a dynamic antibody-antigen binding model.
    Article Snippet: Previously, we have reported novel engineered antibody with pH-dependent antigen-binding (recycling antibody), and with both pH-dependent antigen-binding and increased FcRn-binding at neutral pH (sweeping antibody).. The purpose of this study is to perform PK/PD predictions to better understand the potential applications of the antibodies as therapeutics.. To demonstrate the applicability of recycling and sweeping antibodies over conventional antibodies, PK/PD analyses were performed.

    Article Title: Anti-CD137 antigen-binding molecule and utilization thereof
    Article Snippet: .. Murine plasma samples diluted 50 fold were mixed with a SULFO-TAG (Meso Scale Diagnostics, LLC)-labeled anti-human IL6R antibody (R&D Systems), a biotin-labeled anti-human IL6R antibody (R&D Systems), and excessive anti-human IL6R antibody tocilizumab (in-house manufactured product), and the mixture was incubated overnight at 37° C. After the mixture was added to the blocked Streptavidin-coated standard 96-well plate (Meso Scale Diagnostics, LLC), followed by addition of Read buffer, the reaction was measured on SECTOR Imager 6000 (Meso Scale Diagnostics, LLC). .. SOFTmax PRO (Molecular Devices) was used to calculate concentrations of the soluble antigen in murine plasma.



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    Image Search Results


    Th17 cultures containing IL‐21+ TGF‐β1 (Th17.d) develop independently of T cell–intrinsic IL‐6 . WT or Il6 KO CD4 + T cells were differentiated under Th17.d conditions for 7 days, and Il17A expression was assessed by ELISA (A) and qPCR (B). Responsiveness to CD8 + T‐cell‐mediated suppression was evaluated in coculture assays (C). In parallel, WT cells were cultured with or without anti–IL6R antibody and subjected to the same functional readouts (D–F). Data are from three independent experiments ( n = 6 per group); each point represents an individual donor. Error bars indicate SEM. Statistical analysis was performed using paired or unpaired Student's t ‐test (* p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; ** p ≤ 0.0001).

    Journal: European Journal of Immunology

    Article Title: CD4 + T‐Cell‐Intrinsic IL‐6 Is Critical for Th17 Differentiation and Dampened Responsiveness to CD8 + T Cell‐Mediated Suppression

    doi: 10.1002/eji.70150

    Figure Lengend Snippet: Th17 cultures containing IL‐21+ TGF‐β1 (Th17.d) develop independently of T cell–intrinsic IL‐6 . WT or Il6 KO CD4 + T cells were differentiated under Th17.d conditions for 7 days, and Il17A expression was assessed by ELISA (A) and qPCR (B). Responsiveness to CD8 + T‐cell‐mediated suppression was evaluated in coculture assays (C). In parallel, WT cells were cultured with or without anti–IL6R antibody and subjected to the same functional readouts (D–F). Data are from three independent experiments ( n = 6 per group); each point represents an individual donor. Error bars indicate SEM. Statistical analysis was performed using paired or unpaired Student's t ‐test (* p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; ** p ≤ 0.0001).

    Article Snippet: IL6/STAT3 Pathway Blockade: CD4+ naive T‐cells were isolated, treated with human anti‐IL6R alpha antibody (R&D Systems, MAB227‐100) to block the IL‐6 receptor, and differentiated into Th17 subtypes in the presence of human recombinant cytokines as stated above.

    Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Cell Culture, Functional Assay